Texas red phalloidin
WebTexas Red®-X phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Texas Red®-X dye. • Selectively stains F-actin. • Outstanding … WebOct 1, 1999 · For visualization of intracellular F-actin organization, the cells were probed with Texas Red-conjugated phalloidin. For detection of nontagged CPD, the samples were incubated with the anti-CPD antibody, followed by an FITC-conjugated secondary antibody. Fluorescent images were visualized using a confocal microscope (MRC1024; Bio-Rad). …
Texas red phalloidin
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WebApr 12, 2024 · The tissues were stained using Texas-Red Phalloidin (1:400 dilution, Invitrogen, Waltham, MA, USA) and incubated with mouse primary antibodies against LamC (1:200 dilution, ... Oil Red O is a fat-soluble dye used to stain neutral lipids and fat droplets. To examine the lipid content of the adipose tissue in larvae expressing either wild-type or ... WebRhodamine Phalloidin is conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC) (Figure 5). Alexa Fluor 546, Alexa Fluor 568, Alexa Fluor 594, and Texas Red-X (Figure 6) phalloidins are available …
WebAug 30, 2024 · Actin was stained with Texas-Red-phalloidin (red). Insets magnify the indicated area. Scale bar is 10 μm. (F) Fluorescence microscopy of cells expressing Emerald-vinculin (green) and Apple-actin (purple). Cells were infected with WT Salmonella, and still images taken from a live movie at 0, 6, 13, and 19 minutes post infection. Scale …
WebMay 15, 2006 · The tagged CA domain colocalizes extensively with Texas Red–labeled Arp3, as shown by the merger of fluorescent signals. (J) Phalloidin staining of WASp-CA–expressing cells confirms disruption of actin filaments, which also failed to colocalize with the EGFP-CA fragment (data not shown). WebPhalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications …
WebDec 5, 2003 · Endogenous RhoA staining was visualized with an anti-RhoA primary antibody (green), and actin was detected with Texas red–conjugated phalloidin (red). Superimposition of the images (merge) reveals colocalization of RhoA and F-actin in the protrusions of Smurf1 siRNA-treated HEK293T cells (yellow arrowheads).
WebTexas Red (and Texas Red-X) dye products. We offer a broad range of Texas Red and Texas Red-X dye products including secondary antibodies, labeling kits, bioconjugates, and … prayer on stewardshipWebOct 2, 2024 · F-actin was visualized by Texas Red-phalloidin. Data are representative images of three independent experiments (a, c, and e), and >25 cells were analyzed in three independent experiments (b, d, and f). (g and h) Activities of CDC42, RAC1, and RAC2. Activities of CDC42, RAC1, and RAC2 were measured by GST-PBD pulldown coupled with … scitecs hpWebPhalloidin is a bicyclic peptide that can be found naturally in the death cap mushroom. This molecule is considered to bind so tightly to F-actin that when ingested by an organism, it … scitecs suppoters clubWebLive-or-Dye NucFix™ Red is a unique, cell membrane impermeable dye that specifically stains the nuclei of dead cells. The dye is able to enter into dead cells that have compromised membrane integrity and covalently label the cell nucleus, allowing for clear differentiation of live and dead cells by either microscopy or flow cytometry. prayer on the miraculous medalWebApr 12, 2024 · Actin was visualised with Texas-Red Phalloidin (Red). Scale bar is 2 µm (D) Average percentage of S. Tm associated with an actin-rich ruffle. WT or ∆N-WASP MEFs were pre-infected with unstained WT S. Tm (WT) or treated with PBS as a control (PBS), then infected for 5 minutes with WT S. Tm pre-stained with Alexa-Fluor 350. ... scitec shredexWebThe actin cytoskeleton was stained with Texas Red ®-X phalloidin (Molecular Probes). To that end, the commercial solution was prepared in PBS and left in dark conditions for 20 min at room temperature (RT). Hoechst (Invitrogen, Molecular Probes) was employed as a contrast marker, to detect cell nuclei. Fully-dense and porous substrates were ... scitec sound balatonWebPrepare a 1 mL solution containing 50 to 100 µg/mL lysopalmitoylphosphatidylcholine and 3.7% formaldehyde and then add 5–10 units of fluorescent phallotoxin (approximately 25 … scitec shark cartilage