Webionomycin, and PMA were from Sigma (St Louis, MO). Anti-CD 3-e was purchased from Pharmingen (San Diego, CA). Cell isolation, enrichment, identification, culture, and … WebPMA/ionomycin served as a control (Fig 4C). There were no differences in the abilities of Calhm6 −/− and WT NK cells to respond to soluble signals and produce IFN-γ. Even NK cells purified from mice previously injected with Poly(I:C) in vivo for 3 h, had nearly WT levels of IFN-γ production when stimulated with PMA/Ionomycin for an additional 4 h in …
Pitfalls in determining the cytokine profile of human T cells
Web3 jan. 2024 · Ionomycin is a Calcium ionophore and an antibiotic produced by Streptomyces conglobatus ATCC 31005 [1]. It is observed that LCLC 103H cells overexpressing the catalytic subunit of μ-calpain rapidly underwent cell death after treatment with ionomycin. The first signs of Ionomycin-induced cell death are detected 3 h after … Web1 jun. 2005 · Ionomycin inhibits the transcriptional activity of β-catenin/Tcf in HCT116 cells. HCT116 cells were co-transfected with reporter genes harboring Tcf-4-binding sites (TOPflash) or a mutant Tcf-binding site (FOPflash), respectively, and β-galactosidase gene. sifma press releases
Ionomycin, calcium salt - Biotium
WebIonomycin is commonly used to modify intracellular calcium concentrations and to calibrate fluorescent calcium indicators. It is also known for its antiproliferative effects. Yellowish oily solid soluble in DMSO or EtOH Store desiccated at ≤ 4°C C 41 H 70 O 9 Ca MW: 747.1 [56092-82-1] References 1) J Biol Chem 275, 7071 (2000). WebChemicalBook provide Chemical industry users with Ionomycin, 1 mg Boiling point Melting point,Ionomycin, 1 mg Density MSDS Formula Use,If You also need to Ionomycin, ... Ionomycin, 1 mg: CAS: MF: MW: 0: EINECS: Product Categories: Mol File: Mol File: Ionomycin, 1 mg Chemical Properties: Safety Information: MSDS Information: Web22 mrt. 2013 · Ionomycin Evokes a Biphasic Ca 2+ Response. Murine cerebellar astrocytes in primary culture were treated with 1 μM ionomycin and the cytosolic Ca 2+ concentration ([Ca 2+] c) was monitored by recording changes of the fura-2 340/380 ratio in cell populations plated in 96-well plates.Ionomycin evoked an instant increase in [Ca 2+] c … sifma ops conference 2023